Different ligase enzymes contain the word â€˜ligaseâ€™. One of the most important ligases is DNA ligase. It helps to connect DNA fragments together. Ligase can also be called synthetase as they are used for the synthesis of new molecules. Synthetases use the nucleoside triphosphates like ATP, CTP, UTP, TTP and GTP for the production of energy. It helps to join compounds with the demand for energy. In biochemical nomenclature, sometimes the word â€˜synthetasesâ€™ has been differentiated from â€˜synthasesâ€™ and sometimes both have been considered as synonyms. Unlike synthetases, nucleoside triphosphates are not used by synthases. Also, synthase is an enzyme, called lyase, which catalyzes the cleavage of chemical bonds by reactions apart from oxidation and hydrolysis, without the demand for energy. According to the Joint Commission on Biochemical Nomenclature, any enzyme that helps in the synthesis of other compounds can be called â€˜synthaseâ€™.
On the basis of the chemical reactions which an enzyme catalyzes, it has been assigned a numerical scheme, which is called Enzyme Commission Number (EC number). According to EC number classification, ligases have been classified as EC 6. The six subclasses of ligases are as follows:
One of the most important ligase enzymes is DNA ligase. It is an enzyme that catalyzes the joining of the two DNA strands. This takes place by the formation of bond between the phosphate group of one DNA strand and the deoxyribose group of the other DNA strand. During DNA replication, DNA ligase catalyzes the joining of Okazaki fragments formed on the lagging strand of DNA. It is also useful in the recombination and repair process of DNA. It helps to repair single-strand breaks in the DNA using the complementary strand as the template. In molecular biology, DNA ligase is also used in gene cloning to form recombinant DNA. In nano chemistry, it is used in DNA origami, in which the construction of DNA lattice structure takes place. Another enzyme that is very important in cell biology is E3 ubiquitin ligase. Many important biological processes like DNA repair, signaling and cell trafficking are regulated by the enzyme E3 ubiquitin ligase. E3 ligases play an important role in cell cycle control. It has also been found that E3 ligases can be used as druggable targets for medicinal purposes.
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